Toe separators should not be worn for more than one hour at a time. It is recommended to start with shorter sessions, such as 15-30 minutes, to gradually get your feet accustomed to using them. After the adaptation period, which varies for each person, it is recommended to wear them no longer than one hour a day. The primary purpose of separators is to stretch and align the toes, which can help improve foot positioning, reduce muscle tension, and prevent deformities like bunions. Regular, but short, use of separators can be beneficial, but always monitor how your feet respond and consult a specialist if discomfort occurs.